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Total GLP-1 Elisa Kit for Human/mouse/rat

This ELISA (enzyme-linked immunosorbent assay) kit is produced for the quantitative determination of the total value of glucagon-like peptide-1 (7-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. This kit is for research purpose only.   PRINCIPLE of this ELISA is designed, developed and produced for the quantitative measurement of GLP-1 (7-36) and (9-36) in plasma sample. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 antibodies.   Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidate (HRP) conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36)/(936) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP1 (7-36)/(9-36) on the wall of the microtiter well is directly 

Same Kit can be used for various species. kindly ask for more details..